Title: Development of Biosensors for POCT Diagnosis and Bio-Manufacturing
Abstract: Exosomal microRNAs (miRNAs) play a key role in cell-cell communication to regulate gene expression in target cells and have great potential as biomarkers for disease diagnosis. In this seminar, we would like to share an on-chip nucleic acid amplification and detection system for rapid RNA analysis, such as exosomal miRNAs. Paper-based nucleic acid tests have been demonstrated in lab for the detection of DNA and RNA molecules extracted from virus and bacteria. In clinical samples, these nucleic acids are mostly encapsulated in lipid membranes and need to be released before being analyzed using paper-based sensors. For the nucleic acid amplification tests (NAATs), it is also desirable to remove the interfering molecules that can inhibit the amplification. To achieve a field deployable NAAT, we demonstrated a pump-free sensor system that combines the thermolysis and paper-based NAATs to detect target RNA molecules carried by viral and exosomal nanoparticles. Owing to the advantages of easy storage, simple operation, and low cost, such as system has great potential to be used as a point-of-care test for in-field diagnosis of viral and bacterial infections.
Bio: Jingjing Qian is currently a graduate candidate in the Department of Electrical and Computer Engineering at Iowa State University, working as a research assistant in Dr. Meng Lu’s research group. She received her bachelor’s degree in optoelectrical engineering from Nanjing University, China, in 2017. Her research interests include nanophotonics and sensors, spectroscopy for energy and biomedical applications.
Advisor: Dr. Meng Lu
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